FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n02_bulkATAC_ezh2_NT1rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n02_bulkATAC_ezh2_NT1rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23058473
Sequences flagged as poor quality0
Sequence length38
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAAGAGG2280630.9890637597728175No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG954400.413904251161818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA236350.031.3898428
CCGACGA240000.030.93871327
TGCCGAC242750.030.60034225
TGACGCT245450.030.47218319
TCTGACG247550.030.21381217
ACGAAGA247000.029.9771830
GACGAAG247300.029.94107629
CTGCCGA250350.029.6771424
GCCGACG250600.029.65507526
ACGCTGC252600.029.65218221
CGAAGAG251500.029.390331
ATCTGAC256850.029.23182516
TATACAC257950.029.1265359
GACGCTG259700.028.8864520
TTATACA259900.028.878548
CTGACGC260400.028.8642218
CTTATAC260350.028.786237
TACACAT262500.028.6544511
TCTTATA269750.027.8009686
ATACACA274300.027.44052510
Produced by FastQC (version 0.11.5)