FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n02_bulkATAC_ezh2_E3rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n02_bulkATAC_ezh2_E3rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21260753
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGATACTC494100.23240004716672075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT54250.030.90854628
ACGATAC54550.030.82568730
CGATACT54700.030.65405831
GACGATA55400.030.29497129
CCGACGA56950.029.49894727
TGCCGAC58450.028.7400925
TGACGCT60800.027.6027419
GCCGACG61400.027.41259426
TCTGACG62000.027.1722217
CTGCCGA64600.025.90365624
GAATCGC69650.025.31301125
ACGCTGC66950.025.08899321
CCGAGTA95300.024.9456022
AGGCCGA114100.024.91281511
CGAGTAG96750.024.8627873
CTGACGC69500.024.5621918
AATCGCT72750.024.49937426
GGATTAC480550.024.23519513
GTAGCTG370500.024.2188916
TTGGGAG431500.024.2140166
Produced by FastQC (version 0.11.5)