FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n02_bulkATAC_ezh2_E3rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n02_bulkATAC_ezh2_E3rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27313463
Sequences flagged as poor quality0
Sequence length38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGATATGC778950.28518902930763484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT80900.030.53565428
GACGATA81350.030.38590629
ACGATAT81800.030.14023630
CCGACGA86750.028.66054227
CGATATG86700.028.47397431
TGACGCT90400.027.80268719
TCTGACG91100.027.51885817
TGCCGAC90750.027.39606525
GATATGC95200.025.96522132
CTGCCGA96550.025.70003924
ACGCTGC98800.025.4861221
GCCGACG99600.025.05888726
CTGACGC104750.024.22310318
GACGCTG104550.024.05520620
GAATCGC75450.023.7064425
CTTATAC106050.023.6116127
TATACAC106150.023.588169
ATCTGAC109850.023.06936816
TTGGGAG433450.023.0007676
GGATTAC458900.022.87593713
Produced by FastQC (version 0.11.5)