FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n02_bulkATAC_ezh2_E2rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n02_bulkATAC_ezh2_E2rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20383176
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGATATGC816120.4003890267149732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT85800.030.71222128
GACGATA86250.030.64465929
ACGATAT86950.030.39787930
CCGACGA88400.029.91699827
CGATATG89900.029.400931
TGCCGAC91700.028.94321625
TGACGCT93400.028.64013919
GCCGACG94200.028.1761926
TCTGACG95450.028.02551317
CTGCCGA97500.027.23733924
GATATGC97550.027.19371832
ACGCTGC98900.027.0448221
CTGACGC102850.026.07150518
GACGCTG103050.026.03566620
GAATCGC69450.025.08565125
ATCTGAC109450.024.60137716
TATACAC110200.024.4785219
AGGCTGA396250.024.42861611
CTTATAC110650.024.2784677
TTGGGAG389250.024.2025536
Produced by FastQC (version 0.11.5)