FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n02_bulkATAC_ezh2_E2rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n02_bulkATAC_ezh2_E2rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20868519
Sequences flagged as poor quality0
Sequence length38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGACTCCT813740.3899366313440834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACT87900.030.39788629
ACGACTC88900.030.09187730
CCGACGA88900.030.05573727
CGACGAC90400.029.66350228
TGACGCT93950.028.88095319
TGCCGAC92950.028.81334725
TCTGACG95050.028.49670217
GCCGACG97100.027.5497226
CTGCCGA98100.027.26699424
ACGCTGC101400.026.75723321
CGACTCC101900.026.50409531
CTGACGC104900.026.09535818
GACGCTG106000.025.64300520
CTTATAC106650.025.5347237
TATACAC107900.025.297019
GAATCGC55100.025.20320525
TACACAT111450.024.43023311
ATCTGAC111750.024.39561516
TTATACA112000.024.2718338
ATCGCTT67200.023.80912427
Produced by FastQC (version 0.11.5)