FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n02_bulkATAC_ezh2_E1rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n02_bulkATAC_ezh2_E1rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24204988
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAAGAGG716590.2960505495809376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA79400.030.18658828
CCGACGA81150.029.67307927
TGCCGAC84950.028.34333425
GACGAAG84900.028.32462129
TGACGCT86250.027.99113719
GCCGACG86250.027.8805326
ACGAAGA86550.027.76626630
TCTGACG86900.027.7264217
CGAAGAG89350.026.96793731
CTGCCGA90000.026.69924424
ACGCTGC92950.026.1090821
CTGACGC94750.025.5815318
GAATCGC74250.025.25276825
GACGCTG96350.025.14011620
AGGCCGA118200.024.82048411
AATCGCT79100.024.23156526
TTGGGAG454400.023.757196
CCGAGTA96850.023.7174032
CGAGTAG97050.023.6811033
AGGCTGA462550.023.66553311
Produced by FastQC (version 0.11.5)