FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n02_bulkATAC_ezh2_E1rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n02_bulkATAC_ezh2_E1rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21261804
Sequences flagged as poor quality0
Sequence length38
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAAGAGG784230.36884452514001165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA83300.030.2512328
TGACGCT89450.028.86743219
CCGACGA88000.028.67149427
TCTGACG91200.028.36633717
TGCCGAC89250.028.32211325
GACGAAG92150.027.44978129
CTGCCGA92950.027.28045324
ACGAAGA93850.027.10604930
GCCGACG96250.026.3629226
TATACAC99150.026.01239
ACGCTGC99400.025.9605321
CTTATAC100250.025.6318457
CGAAGAG98900.025.62508431
TTATACA100750.025.5521018
ATCTGAC103800.025.1079816
CTGACGC104350.025.00630618
GACGCTG103900.024.89891820
TACACAT104550.024.7570311
TCTTATA111950.022.9959686
GAATCGC46050.022.96405825
Produced by FastQC (version 0.11.5)