FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39837469
Sequences flagged as poor quality0
Sequence length38
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGA46500.027.64917217
ATCTCCG47650.027.5523916
TCCGAGC48600.025.6092519
CGAGTAG29850.024.544983
ACGAGAC48350.024.17677728
CCGAGTA30900.023.555972
CCCGAGT32000.023.4308171
GTAGCTG113350.023.3136566
AGTAGCT115100.023.2509885
CTCCGAG54100.023.11500418
AGGCTGA125550.023.11362611
TTTGGGA92350.022.6564395
TATACAC72050.022.61269
GATTACA152550.022.6074214
TCTTATA72450.022.0792066
CTGGGAT165200.022.04711710
CGAGCCC55000.021.94827821
ATTACAG154300.021.91641415
TTACAGG144100.021.75059316
AATCGCT21500.021.74889426
Produced by FastQC (version 0.11.5)