FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n01_bulkATAC_ezh2_NT3rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n01_bulkATAC_ezh2_NT3rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22582024
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCCT1178850.5220302661975738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG135900.029.62149629
ACGAGAC136600.029.48606128
GACGCCT139150.028.9172932
TCTCCGA140600.028.8499817
AGACGCC140200.028.80680531
GAGACGC142400.028.37827530
ATCTCCG143800.028.2079816
TCCGAGC146050.027.80965819
CCCACGA146400.027.63395925
CGAGCCC150750.027.00067521
CACGAGA149450.026.962727
CCACGAG152350.026.4812226
GCCCACG156800.025.89126424
TATACAC159150.025.5169649
CTCCGAG159650.025.49766718
CTTATAC159800.025.4448367
GAATCGC72250.025.37152525
CCGAGCC164000.024.97738620
TACACAT163200.024.94242511
TTATACA166400.024.4436458
Produced by FastQC (version 0.11.5)