FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n01_bulkATAC_ezh2_NT3rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n01_bulkATAC_ezh2_NT3rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20746298
Sequences flagged as poor quality0
Sequence length38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT626780.3021165511070939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC71500.028.52375228
TCTCCGA75350.027.36290417
CCCACGA80650.025.6057225
ATCTCCG81800.025.20531316
GAATCGC57150.025.0702425
CACGAGA83250.024.4983527
TCCGAGC84150.024.4475819
CTTATAC84500.024.2870047
ATCGCTT70600.023.85408227
TATACAC86600.023.7337999
CCGAGTA83400.023.7282242
CGAGTAG83800.023.5766683
AGAATCG62850.023.406424
CCACGAG88100.023.27688626
TTGGGAG331850.023.19756
CGCTTGA70000.023.11688829
GGATTAC368250.023.05961413
GTAGCTG282650.023.0584146
GGGATTA393400.023.03691112
CGAGCCC89700.023.01753621
Produced by FastQC (version 0.11.5)