FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n01_bulkATAC_ezh2_NT2rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n01_bulkATAC_ezh2_NT2rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25543458
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCCT1240850.48577995978461486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC137700.029.19175328
CGAGACG139550.028.73193429
GACGCCT141650.028.32844532
TCTCCGA144600.027.9635417
AGACGCC144800.027.74864431
CCCACGA147150.027.44899725
ATCTCCG147300.027.40753616
GAGACGC146750.027.38523330
TCCGAGC149100.027.12360619
CCACGAG151550.026.51478626
CACGAGA153900.026.04724727
CGAGCCC156050.025.98983221
GCCCACG160450.025.32078424
GAATCGC77800.024.8163125
TATACAC163450.024.7773289
AGGCCGA124600.024.4027611
CTTATAC166500.024.2582027
AATCGCT81500.024.10223626
TTATACA168300.024.0633078
TACACAT169750.023.97078111
Produced by FastQC (version 0.11.5)