FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n01_bulkATAC_ezh2_NT2rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n01_bulkATAC_ezh2_NT2rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23180423
Sequences flagged as poor quality0
Sequence length38
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT1581190.682123013889781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC169500.030.04131928
TCTCCGA177250.028.76934817
TATACAC179450.028.4698819
ATCTCCG180150.028.3683816
CTTATAC181600.028.1255917
CCCACGA183300.027.96451425
TACACAT185900.027.53371411
TTATACA185500.027.5154828
TCCGAGC186700.027.39369419
CACGAGA186000.027.30892627
CCACGAG192100.026.51680826
TCTTATA192800.026.4525956
CGAGACC195400.026.07160229
ATACACA197100.026.001610
CGAGCCC198750.025.84175321
AGACCTC198950.025.55183231
GACCTCT199200.025.53313332
GCCCACG202250.025.44571324
ACATCTC203450.025.30792614
CTCTTAT203800.025.0561715
Produced by FastQC (version 0.11.5)