FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n01_bulkATAC_ezh2_NT1rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n01_bulkATAC_ezh2_NT1rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28029798
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCCT550180.1962839689390555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC67800.027.48431828
CGAGACG70550.026.24871629
TCTCCGA73350.025.51308417
TCCGAGC73650.025.34735519
GACGCCT73800.025.08323932
GAATCGC88900.025.0686525
ATCTCCG75650.024.69511816
AGGCCGA136850.024.56707811
AGACGCC75950.024.5236631
CCCACGA77250.024.47624825
GAGACGC76650.024.26171530
TTGGGAG523850.023.9175856
AATCGCT95350.023.75905626
AGGCTGA533700.023.66331311
CGAGTAG118700.023.5852073
CCGAGTA119500.023.574532
ATCGCTT112650.023.39316727
AGAATCG98250.023.3816324
CCCGAGT123550.023.192681
CGAGCCC81300.023.09281321
Produced by FastQC (version 0.11.5)