FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n01_bulkATAC_ezh2_NT1rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n01_bulkATAC_ezh2_NT1rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23058473
Sequences flagged as poor quality0
Sequence length38
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT2285150.9910239936530056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC244150.030.6675228
TCTCCGA250300.030.02140817
CCCACGA253950.029.51723725
ATCTCCG256450.029.35135316
TATACAC257750.029.2216789
TTATACA260200.028.9280918
CTTATAC260350.028.839397
CACGAGA259950.028.74343327
TACACAT263350.028.65489411
TCCGAGC262900.028.64068419
CCACGAG265700.028.16972526
CGAGACC267050.027.9956229
CGAGCCC270700.027.7254121
GCCCACG271700.027.66273524
TCTTATA273550.027.4500246
ATACACA275850.027.33901610
GACCTCT274100.027.2943932
AGACCTC275700.027.16901231
ACATCTC283000.026.65965814
GAGACCT283050.026.50676530
Produced by FastQC (version 0.11.5)