FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n01_bulkATAC_ezh2_E3rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n01_bulkATAC_ezh2_E3rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21260753
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCT496870.23370291729554452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC58600.028.71191628
CGAGACG60900.027.4647329
TCTCCGA63750.026.64521417
GAGACGT63400.026.50571830
AGACGTC65300.026.41639731
GACGTCT64450.026.14125632
ATCTCCG66150.025.60595716
GAATCGC71050.025.23705325
CCCACGA67500.025.02265525
TCCGAGC67850.024.94422319
AGGCCGA110250.024.8801111
AATCGCT76250.024.20879226
TTGGGAG419200.024.1870256
CGAGTAG101900.024.098263
AGGCTGA419950.024.07437111
GGATTAC479200.023.97823713
GGGATTA515000.023.9481112
AGTAGCT375850.023.9249255
ATCGCTT90600.023.8907127
GTAGCTG374950.023.811696
Produced by FastQC (version 0.11.5)