FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n01_bulkATAC_ezh2_E3rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n01_bulkATAC_ezh2_E3rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27313463
Sequences flagged as poor quality0
Sequence length38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC780750.2858480449732793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC89550.027.81546628
TCTCCGA94300.026.51202617
ATCTCCG97650.025.60249516
CCCACGA101450.024.75895725
TCCGAGC102800.024.33824719
GAATCGC74950.024.20024125
CACGAGA105350.023.7056327
ATCGCTT89650.023.46424327
CTTATAC106950.023.3621357
TATACAC107300.023.3442789
CCGAGTA99100.023.0839672
CCACGAG109250.022.9766626
TTGGGAG433650.022.9603396
AGGCTGA449100.022.77996411
GGATTAC459750.022.68019913
CGAGCCC111100.022.67553121
CGAGTAG100650.022.66493
GGGATTA498500.022.64986612
CGCTTGA89750.022.61253229
TTATACA110900.022.514378
Produced by FastQC (version 0.11.5)