FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n01_bulkATAC_ezh2_E2rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n01_bulkATAC_ezh2_E2rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20383176
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCT818600.40160571640062376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC92800.028.65255228
CGAGACG92800.028.6479829
GAGACGT95300.027.99454730
TCTCCGA96150.027.91488317
AGACGTC97850.027.7843631
GACGTCT97900.027.32577132
TCCGAGC99950.026.8890919
ATCTCCG100650.026.68271816
CCCACGA100550.026.63628425
CACGAGA104000.025.53704827
CGAGCCC105850.025.50905821
GAATCGC66850.025.36029625
TATACAC108800.024.7865129
CCACGAG108000.024.6358826
GCCCACG110050.024.52458624
CGAGTAG91000.024.4526983
CCGAGTA91350.024.4116062
AGGCCGA106650.024.40132511
CTTATAC110800.024.238887
AGGCTGA391450.024.16527611
Produced by FastQC (version 0.11.5)