FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n01_bulkATAC_ezh2_E2rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n01_bulkATAC_ezh2_E2rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20868519
Sequences flagged as poor quality0
Sequence length38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC816290.3911585676012754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC91100.029.3463528
TCTCCGA96300.028.05327217
ATCTCCG99800.027.21368416
CCCACGA99800.026.88582825
TCCGAGC102800.026.29831119
TATACAC106050.025.579579
CACGAGA105050.025.40483527
CTTATAC106850.025.254727
CGAGACT108100.024.90445929
CCACGAG109150.024.56806826
TTATACA110500.024.477078
CGAGCCC110850.024.47444721
GAATCGC55900.024.45828425
GCCCACG112300.024.01953924
TACACAT113550.023.94632311
TCTTATA115600.023.414746
GGATTAC368750.023.37942313
TTGGGAG327300.023.2261476
GGGATTA396600.023.08871812
GATTACA378300.023.02599314
Produced by FastQC (version 0.11.5)