FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n01_bulkATAC_ezh2_E1rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n01_bulkATAC_ezh2_E1rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24204988
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCT717590.2964636875672072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG84750.028.51826929
ACGAGAC85800.028.1921228
GAGACGT87450.027.68947430
AGACGTC89250.027.5921331
GACGTCT87750.027.55036432
TCTCCGA89400.027.069917
TCCGAGC91850.026.29896519
ATCTCCG91750.026.23709116
CCCACGA93850.025.87761725
CGAGCCC96900.024.97491621
GAATCGC79900.024.44477525
CCACGAG100300.024.26138926
AGGCCGA116400.024.21191811
AGGCTGA461300.023.82042911
AATCGCT84150.023.78093126
TTGGGAG451100.023.7463056
CACGAGA101850.023.71916427
CCGAGTA101800.023.6347892
CGAGTAG102750.023.634243
GGATTAC512000.023.55470513
Produced by FastQC (version 0.11.5)