FastQCFastQC Report
Mon 9 Dec 2019
HVHW3BGXC_n01_bulkATAC_ezh2_E1rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHW3BGXC_n01_bulkATAC_ezh2_E1rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21261804
Sequences flagged as poor quality0
Sequence length38
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC785740.3695547188752187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC88900.028.55931728
TCTCCGA92750.027.45567917
CCCACGA98200.025.95358525
TATACAC99100.025.6952029
TTATACA99050.025.6597278
ATCTCCG100450.025.43068316
CTTATAC100800.025.1680627
CGAGACT101200.025.10015329
TCCGAGC102150.024.93192119
CACGAGA102700.024.72267527
TACACAT103650.024.644411
TCTTATA109050.023.3389476
CCACGAG109900.023.2487726
CGAGCCC110500.023.233421
GAATCGC47350.023.02464325
GGATTAC319450.022.95236413
AGACTTC111500.022.89055831
TTGGGAG286200.022.83286
GATTACA326100.022.75418714
GGGATTA345400.022.6357312
Produced by FastQC (version 0.11.5)