FastQCFastQC Report
Sun 14 Feb 2021
HVHVCDRXX_n02_E140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHVCDRXX_n02_E140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29612353
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC2169670.7326908469583623No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC1691390.5711771705544642No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA1536160.5187564797704525No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC990410.334458393090208No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT899900.3038934460898801No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA811960.27419638013905884No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA719440.24295266235682114No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA599040.2022939548235157No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC594360.200713533301457No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC584830.19749528178324768No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC583290.19697522854735658No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC556310.18786416601206934No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC550430.18587850820230328No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA501870.16947994642641198No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG491010.16581255802266034No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG451750.1525545774765011No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC451380.1524296296211247No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG425750.14377445790950824No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT418460.141312647461686No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT410510.13862795705562472No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT399280.13483562079649664No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC397000.13406567184985266No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA387990.13102302272298322No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC380290.12842275654352764No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC373820.1262378575589721No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC371370.12541050013823624No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC363860.12287439637100098No Hit
CTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAG359160.12128722091081381No Hit
CAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGT357700.12079418342743652No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC353670.11943326489455262No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG332940.11243280802440792No Hit
CGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCG323690.109309111639997No Hit
CAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATG308590.10420988835301269No Hit
CGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAG308480.10417274169330618No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA307560.10386206053939719No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT305210.10306847280930359No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC304610.10286585466544992No Hit
GTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGC304030.1026699904597247No Hit
CGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTG302910.10229176992453115No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT301200.10171430821454817No Hit
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT296430.1001034939709114No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)