FastQCFastQC Report
Sun 14 Feb 2021
HVHVCDRXX_n01_E366.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHVCDRXX_n01_E366.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28341156
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1934550.6825938927826374No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC1554020.5483262574046027No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA1384810.4886215650483699No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT1274550.4497170122489005No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC1203330.42458747977676No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA1158550.40878713627630436No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG1084930.38281077878404113No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC971410.34275595533223835No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA950550.3353956345323387No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC937690.3308580637995147No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC909720.32098902387750167No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA888150.31337818400914913No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT886910.3129406577487524No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGTCATATCTCGTAT874910.30870653264813896TruSeq Adapter, Index 6 (97% over 36bp)
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT676390.238659989733658No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA641590.22638102694187914No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC585250.20650180959449924No Hit
GTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAA557960.19687270342818763No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC533090.18809747915716635No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC498610.17593142636807052No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG497610.1755785826096861No Hit
CAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCA492510.1737790794419254No Hit
CTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTT482530.17025769873324856No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT430190.15178985641940645No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACA426820.15060077295365087No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA406490.143427459345695No Hit
GGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGG401990.14183966243296497No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC398930.14075996053230855No Hit
GTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCA397810.14036477552291798No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT396790.14000487488936583No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC396740.1399872327014466No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA388280.13700217450551416No Hit
CTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTG381170.13449345538340074No Hit
CTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTG377560.13321968941563286No Hit
CTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGG374650.1321929140787341No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC371550.1310990984277423No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC370520.13073566935660635No Hit
GTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAG369220.13027697247070658No Hit
CTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGA365100.12882325618616264No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC364610.12865036274455424No Hit
GAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCA364570.12863624899421888No Hit
CAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCC361230.1274577508412148No Hit
CAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACC360110.1270625658318242No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC353980.12489963359292754No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT343900.1213429685084123No Hit
CTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAA331940.11712295715813428No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG329240.11617027901049624No Hit
CGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAG326160.11508352023467214No Hit
CGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGC319650.11278650736758937No Hit
CAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGT317750.11211610422665892No Hit
CGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACC313690.11068355856761805No Hit
TTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAG312600.11029895887097901No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT309060.10904989196629807No Hit
CTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGC307420.10847122820254755No Hit
CGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCAC303920.10723627504820199No Hit
GTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCC300190.105920167829428No Hit
ACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCT299450.1056590634482235No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG299300.10560613688446582No Hit
CTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTC299080.10552851125762125No Hit
CTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGC297580.10499924562004458No Hit
GGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACA296700.10468874311266627No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT296590.10464993029924398No Hit
GCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCA295020.10409596559858038No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC293940.10371489433952517No Hit
CAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATG292860.10333382308046997No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292750.10329501026704768No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT292710.1032808965167123No Hit
CTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAG289850.10227176336773279No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG284920.10053224363889743No Hit
CTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGG283560.1000523761274946No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)