FastQCFastQC Report
Wed 17 Feb 2021
HVHT2DRXX_l01_n01_mb402.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHT2DRXX_l01_n01_mb402.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63377768
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC28844864.551258416042673TruSeq Adapter, Index 22 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCGCGTATGC6145330.9696349672648618TruSeq Adapter, Index 22 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGGATGC1434630.2263617109393944TruSeq Adapter, Index 22 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTTTGC1145340.18071636729144516TruSeq Adapter, Index 22 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATTC1097700.17319953583723555TruSeq Adapter, Index 22 (97% over 48bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)