FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n02_wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n02_wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7157810
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3366894.703799067033073No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG1702612.3786744828376274Illumina Single End PCR Primer 1 (100% over 58bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG319100.061.19030418
GAGCACA403850.061.18822516
AGAGCAC410450.061.13726815
ACACGAG103700.060.97849320
CACACGA104700.060.5608319
AGCACAC343400.060.14533617
CACGTAG99700.060.10320321
AAGAGCA477850.057.8202114
ACACGTA103150.057.72507520
CACGAGA109350.057.48076621
CACACGT226150.057.15839819
AAGGGGG222850.056.4420967
ACGTCAG102350.055.85057422
CACGTCA104850.054.15697521
CGTCAGA105250.053.82003823
ACGAGAT116750.053.748822
ACGTAGA111700.053.46097622
AAAGGGG236300.053.31704766
AGGGGGG243400.052.15864668
CGTAGAT117250.051.54828323
Produced by FastQC (version 0.11.2)