FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n02_triple.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n02_triple.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8223066
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG4259035.179369836019801Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3593264.369732652030277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG229350.063.83764620
CACACGA230200.063.7068319
CACGTAG191050.062.3338521
CACGAGA233200.062.25115621
AGAGCAC805500.061.4459315
GAGCACA794250.061.3871416
AAGGGGG526550.061.10975367
ACACGTA194000.060.88807720
GCACACG618700.060.57171618
AGCACAC659050.060.26572417
AAAGGGG536900.059.93171366
AAGAGCA928500.059.66291414
AGGGGGG556500.058.12467668
ACGAGAT249100.058.0837922
ACGTAGA207000.057.24751322
ACGTCAG162300.057.1361522
AAAAGGG571100.056.5555465
CACACGT399000.056.33890519
GCACAAG139300.055.92075718
CACGTCA166000.055.7171621
Produced by FastQC (version 0.11.2)