FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n02_ds774-5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n02_ds774-5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12618448
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG3198252.534582699869271Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1878711.488859802726928No Hit
TTTGGAAAAAAAATATACGCTAAGATTTTTGGAGAATAGCTTAAATTGAAGTTTTTCTCGGCGAGAAATACGTAG146250.11590173371558848No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG413350.058.47368666
AAGGGGG449850.053.62187667
GCACACG52450.053.0787718
AAAAGGG486550.049.9469365
AGGGGGG486550.049.5701468
AGCACAC60050.048.773917
GAGCACA72650.046.10801316
CACACGT40150.045.88265219
GTGACTG54300.044.0526851
CACGTAG32000.043.55361621
GATCGCC23600.043.12888744
AAAAAGG580800.042.48919764
TGACTGG57200.042.0514532
AGAGCAC81300.041.83879515
GACTGGA60500.041.2888533
CGCCGTA613850.040.087547
GCCGTAT614900.040.08077248
CCGTATC597900.039.8584949
ACACGTA35150.039.2579420
TCGCCGT623650.039.1145646
Produced by FastQC (version 0.11.2)