FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n02_ds774-05-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n02_ds774-05-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11871411
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG6407745.397622911042335Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1855591.563074515742063No Hit
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA180240.15182693952723902Illumina Single End PCR Primer 1 (100% over 58bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG773450.062.05220466
AAGGGGG799900.059.96153667
AGGGGGG831200.057.9152768
AAAAGGG836050.057.58997365
GCACACG92950.056.1546618
AAAAAGG905500.053.58465664
AGCACAC103700.052.46252417
GTGACTG76750.051.4971471
GAGCACA123150.051.46014416
CACGTAG50000.050.71233421
CACACGT69400.050.70338419
AGAGCAC130250.049.6614915
TGACTGG78950.049.177122
ACACGTA51950.048.4103520
GACTGGA86950.046.5871473
CACACGA35250.046.5847819
AAAAAAG1108150.044.4143963
ACACGAG37300.044.20947320
AGATAGA138550.041.3337827
CCGTATC1203450.040.28935249
Produced by FastQC (version 0.11.2)