FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n02_ds774-023-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n02_ds774-023-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9048748
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG1371861.5160771412796556Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG958671.0594504344689453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG193350.054.6164766
AAGGGGG212850.049.58043367
AGGGGGG228450.046.52699768
CGCCGTA245950.043.3888747
AAAAGGG245650.043.367865
CCGTATC246950.043.29749
GCCGTAT249500.042.81348
GCACACG22350.042.13905718
TCGCCGT251000.041.9798246
CGTATCA262850.040.80919650
GTCGCCG259400.040.70021445
GGTCGCC260150.040.4767844
GTATCAT271950.039.36403751
GTGGTCG272350.038.7497242
CTCGGTG274350.038.42823838
TCTCGGT276500.038.11695537
ATCTCGG277350.038.0374536
TGGTCGC282400.037.27297243
TCGGTGG283800.037.24610539
GGTGGTC284000.037.1469941