FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n02_ds774-014-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n02_ds774-014-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8183193
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG1180161.4421754442306323Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG656080.8017408363703509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG163150.054.51235666
AAGGGGG185550.047.9129167
AGGGGGG202400.044.17978368
CGCCGTA204800.044.0217147
CCGTATC205350.044.0214249
GCCGTAT209500.043.0999948
AAAAGGG210100.042.6111965
TCGCCGT208950.042.1896646
GTCGCCG212350.041.497945
CGTATCA220000.041.29387750
GGTCGCC214150.041.1007644
GTATCAT226550.039.95779451
CTCGGTG223550.039.32021738
GTGGTCG225350.039.2080342
GCACACG20000.039.1549818
TCTCGGT226050.038.9616637
ATCTCGG226650.038.87373736
TCGGTGG231200.038.28846439
GATCTCG231400.038.10558335
GGTGGTC235500.037.5014641
Produced by FastQC (version 0.11.2)