FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n02_ds774-005-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n02_ds774-005-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6180164
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG1700162.7509949574153696Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1193691.9314859605667423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA82300.062.37292519
CACGTAG60400.059.74310321
CACGAGA85950.059.56361421
AGAGCAC301300.059.45826315
GAGCACA296500.059.3969416
ACACGAG87050.059.04872520
ACACGTA60950.058.63799320
GCACACG207400.057.751618
AGCACAC225350.057.5144117
AAGAGCA391400.054.76127614
GCACAAG77050.054.0415318
ACGTCAG49350.053.40712722
AAAGGGG241250.052.46587866
AAGGGGG243650.052.0481967
CACACGT131000.051.32554619
AGCACAA82750.050.6108617
GAGCAAG62600.050.09363616
ACGAGAT102300.050.0439222
CGTAGAT72800.049.85140223
GTGACTG36900.049.580761
Produced by FastQC (version 0.11.2)