FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n02_ds395-5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n02_ds395-5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13222632
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1456341.1013994793169772No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG1253140.9477235697098731Illumina Single End PCR Primer 1 (100% over 58bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG211350.043.72971366
AAGGGGG225250.041.04650567
CACACGA39650.040.54485319
AGGGGGG233650.039.96949468
ACACGAG42550.037.7004420
GCACACG109950.036.52168318
CACGTAG33100.036.2697321
GAGCACA152850.036.1793816
AGAGCAC157950.035.60090315
AGCACAC125100.034.60823417
CACGAGA48050.033.16970421
ACACGTA37800.032.2163220
AAAAGGG295600.031.65212865
GGGGGAT41650.029.6713311
CACACGT86000.027.39777619
CGGGGAC16900.026.5537361
CGGGGAG20650.026.0779571
GTGACTG45150.025.9950221
GAGGGTC27850.025.533541
ACGTCAG37200.025.50250222
Produced by FastQC (version 0.11.2)