FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n02_ds395-023-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n02_ds395-023-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16456272
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG296496118.017209487057578Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2358371.4331131619603759No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGG316750.19247980344515453Illumina Single End PCR Primer 1 (100% over 58bp)
GTGACTGGAGTTCAGACGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATC277190.16844033691227273Illumina Single End PCR Primer 1 (100% over 55bp)
GTGACTGGAGTTCAGACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT262920.1597688711027625Illumina Single End PCR Primer 1 (100% over 57bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA243570.14801043638559208Illumina Single End PCR Primer 1 (100% over 58bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGG221890.13483612813400264Illumina Single End PCR Primer 1 (100% over 58bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA202540.1230776934168322Illumina Single End PCR Primer 1 (100% over 58bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG185540.11274728565497702Illumina Single End PCR Primer 1 (98% over 58bp)
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA165090.10032041278851006Illumina Single End PCR Primer 1 (100% over 58bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1794850.065.95740515
GAGCACA1760450.065.94897516
CACACGA488800.065.0509319
AAGAGCA2456950.064.9760414
AGCACAC1157400.064.7278917
GCACACG1052300.064.5638618
ACACGAG495550.064.0395420
GAGCAAG575800.063.73579816
AGCACAA581000.063.7116217
GCACAAG581200.063.66595518
AAGGGGG3473200.063.01787667
ACACGTA303950.062.81048220
CACGAGA499850.062.7363621
AGAGCAA597800.062.66557315
AGGGGGG3497350.062.58469868
CACGTAG311850.061.98262421
CACACGT575100.061.40192819
AAAGGGG3598100.060.93102666
CACAAGA607450.060.84090419
AGCAAGA607050.060.48887317
Produced by FastQC (version 0.11.2)