FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n02_ds131-05-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n02_ds131-05-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9438473
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2249392.383213894874732No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG917380.9719580699123683Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG51250.052.43696618
GAGCACA79050.048.70306416
AGAGCAC82450.047.48941815
AGCACAC63200.046.506817
AAAGGGG149050.046.31537266
AAGGGGG160400.042.82298767
CACACGT38600.040.66474519
ACACGAG28050.038.2491220
AGGGGGG183550.038.00465868
CACACGA28500.037.76623519
CACGTAG24950.037.1942421
AAAAGGG201800.034.44872765
CCGTATC199150.033.8705749
GCCGTAT200150.033.68410548
CACGAGA31800.033.4131621
ACACGTA27650.033.3127220
CGCCGTA204050.033.00648547
CGTATCA205750.032.96854450
TCGCCGT212500.031.5152246
GTATCAT217450.031.19151351
Produced by FastQC (version 0.11.2)