FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n02_ds131-005-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n02_ds131-005-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10346563
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG2345322.266762402161955Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2260672.1849477937746093No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG162050.061.2043418
AGAGCAC241500.059.7107515
GAGCACA238150.059.1745416
AGCACAC181000.058.55122817
CACACGT112250.055.31966419
ACACGAG66350.054.0736620
CACACGA66250.053.94699519
AAGGGGG330600.053.68913767
AAAGGGG338250.052.6992666
CACGTAG52400.050.82523721
AAGAGCA345900.049.30844514
CACGAGA71650.049.25528321
AGGGGGG363950.049.08222268
ACACGTC73300.048.2406620
ACGTCAG61300.048.22960722
AAAAGGG393100.045.59286565
ACACGTA58450.045.21032720
GCACAAG75700.041.60717818
AAAAAGG461000.039.24426364
CACGCGT9650.038.2516219
Produced by FastQC (version 0.11.2)