FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n02_dna2-frb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n02_dna2-frb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7584019
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3843235.067537409914189No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG3629724.786011216480338Illumina Single End PCR Primer 1 (100% over 58bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG120350.061.14230320
CACACGA120950.060.95308719
AAGGGGG448450.060.40486567
CACGTAG106100.058.3966621
AAAGGGG463800.058.36850766
AGGGGGG465650.058.34405568
AGAGCAC451350.058.34167515
GAGCACA447400.057.9854316
CACGAGA126400.057.94286321
GCACACG340900.056.7414718
AGCACAC370850.056.5869917
ACACGTA109550.056.52611520
AAGAGCA533450.055.72620414
AAAAGGG499200.054.41777865
ACGTAGA118150.052.26530522
ACGAGAT140500.052.12761322
ACGTCAG89500.051.88194322
CACACGT231450.051.11017219
CACGTCA90450.051.10851321
GCACAAG87550.049.9249418
Produced by FastQC (version 0.11.2)