FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n01_wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n01_wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7157810
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1462292.042929331736942TruSeq Adapter, Index 8 (100% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT177100.045.149781
AAAAAAG306350.039.2125969
GAAAAAA361750.033.646164
GCTTGAA360700.032.20413260
CTGCTTG358050.031.4117158
TGCTTGA361950.031.3399259
CTTGAAA392100.029.9243361
TCTGCTT388100.029.20178457
ACGTGTG277300.029.1898772
CTTCTGC388650.028.92078855
TTCTGCT386050.028.71341556
TGCCGTG23250.028.63841869
CGTCTTC399300.028.27057852
GTCTTCT399900.027.81405653
GCCGTCT415150.027.22428950
CCGTCTT422450.026.95801551
TCTTCTG415900.026.86845854
AGACGTG61200.026.7285331
TGAAAAA460850.026.44087263
CGTATGG29050.026.0083469
Produced by FastQC (version 0.11.2)