FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n01_triple.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n01_triple.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8223066
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG4082884.965155332573033TruSeq Adapter, Index 11 (100% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG610250.050.1966769
GACGTGT297950.046.766971
GAAAAAA715250.043.57052664
GCTTGAA825300.037.28381360
CTTGAAA841600.036.7092861
TGCTTGA857150.035.79779459
CTGCTTG862750.035.54544458
TGAAAAA901350.034.4406363
TTGAAAA902100.034.29344662
TCTGCTT905100.033.9165657
TTCTGCT912100.033.61466256
CTTCTGC917850.033.5055655
GTCTTCT946850.032.49393553
TCTTCTG952300.032.30434854
CGTCTTC958550.032.13330552
CCGTCTT1045450.029.41281751
GCCGTCT1050550.029.24704250
ACGTGTG502900.027.990652
TGCCGTC1127700.027.27368249
ATGCCGT1147100.026.81242448
Produced by FastQC (version 0.11.2)