FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n01_ds774-5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n01_ds774-5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12618448
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG3146502.4935713171699088TruSeq Adapter, Index 1 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA151820.12031590572786764TruSeq Adapter, Index 1 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG540600.043.75950269
GCTTGAA560600.042.75220560
TGCTTGA560650.042.38533459
CTGCTTG566600.041.885658
GAAAAAA618550.039.16519564
TGAAAAA624150.038.6424563
CCGTCTT618850.038.54443751
CTTGAAA625250.038.5137761
TTGAAAA624200.038.4790762
TGCCGTC619700.038.4748749
ATGCCGT624900.038.1767948
GCCGTCT626700.038.02860350
CGTCTTC630400.037.87122752
TATGCCG635100.037.51989747
GTATGCC637450.037.41946446
TCTGCTT676150.035.2370557
TCTTCTG682000.035.00589454
CTTCTGC685350.034.9804955
GATCTCG667700.034.91297540
CTCGTAT686050.034.53230743