Basic Statistics
Measure | Value |
---|---|
Filename | HVHNYAFXX_n01_ds774-5-2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 12618448 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG | 314650 | 2.4935713171699088 | TruSeq Adapter, Index 1 (100% over 63bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA | 15182 | 0.12031590572786764 | TruSeq Adapter, Index 1 (100% over 40bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAAAAAG | 54060 | 0.0 | 43.759502 | 69 |
GCTTGAA | 56060 | 0.0 | 42.752205 | 60 |
TGCTTGA | 56065 | 0.0 | 42.385334 | 59 |
CTGCTTG | 56660 | 0.0 | 41.8856 | 58 |
GAAAAAA | 61855 | 0.0 | 39.165195 | 64 |
TGAAAAA | 62415 | 0.0 | 38.64245 | 63 |
CCGTCTT | 61885 | 0.0 | 38.544437 | 51 |
CTTGAAA | 62525 | 0.0 | 38.51377 | 61 |
TTGAAAA | 62420 | 0.0 | 38.47907 | 62 |
TGCCGTC | 61970 | 0.0 | 38.47487 | 49 |
ATGCCGT | 62490 | 0.0 | 38.17679 | 48 |
GCCGTCT | 62670 | 0.0 | 38.028603 | 50 |
CGTCTTC | 63040 | 0.0 | 37.871227 | 52 |
TATGCCG | 63510 | 0.0 | 37.519897 | 47 |
GTATGCC | 63745 | 0.0 | 37.419464 | 46 |
TCTGCTT | 67615 | 0.0 | 35.23705 | 57 |
TCTTCTG | 68200 | 0.0 | 35.005894 | 54 |
CTTCTGC | 68535 | 0.0 | 34.98049 | 55 |
GATCTCG | 66770 | 0.0 | 34.912975 | 40 |
CTCGTAT | 68605 | 0.0 | 34.532307 | 43 |