FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n01_ds774-05-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n01_ds774-05-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11871411
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG6225435.244052286623722TruSeq Adapter, Index 2 (100% over 63bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTT167240.14087626146546522TruSeq Adapter, Index 2 (100% over 62bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG910450.052.2771769
GAAAAAA1000750.048.50810664
GCTTGAA1013400.047.126460
CTTGAAA1053700.045.51389361
TGAAAAA1086000.044.4461263
TGCTTGA1073500.044.2373559
TTGAAAA1085750.044.21486362
CTGCTTG1099600.043.1216358
CGTCTTC1209100.039.430452
CCGTCTT1212200.039.33793651
GCCGTCT1214200.039.23904450
TGCCGTC1225700.038.9806649
TCTGCTT1224450.038.83465657
ATGCCGT1234150.038.7584948
CTTCTGC1231050.038.7105355
GACGTGT42650.038.43451
TCTTCTG1242200.038.3241854
TATGCCG1254100.038.18044347
GTCTTCT1248350.038.11879353
GTATGCC1259500.038.00579546
Produced by FastQC (version 0.11.2)