FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n01_ds774-023-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n01_ds774-023-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9048748
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1339121.4798953402172323TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG234900.044.69262758
TGCTTGA237900.044.2885659
GCTTGAA251050.042.36724560
TGCCGTC253550.041.8542649
ATGCCGT254450.041.7062248
TATGCCG255800.041.39170547
GTATGCC258650.040.85558746
CCGTCTT259300.040.76647651
GCCGTCT260250.040.6044150
CGTCTTC274100.038.67857452
CATCTCG273800.038.1161440
CTCGTAT280550.037.5679843
GCATCTC279050.037.374339
AGGCATC282200.037.09159537
CGTATGC289950.036.6118245
GGCATCT285450.036.52425438
TAGGCAT288800.036.3633936
ATCTCGT292750.035.70776441
AAAAAAG299550.035.49606769
CTTAGGC298500.035.48223534
Produced by FastQC (version 0.11.2)