FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n01_ds774-014-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n01_ds774-014-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8183193
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1159481.416904135097388TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG200200.043.73636658
TGCTTGA201450.043.6876259
TGCCGTC210450.041.73732849
ATGCCGT211500.041.57905648
GTATGCC213350.041.21851346
TATGCCG214000.041.12556547
GCTTGAA215000.041.0305660
CCGTCTT215150.040.7774651
GCCGTCT219050.040.08295450
CGTCTTC230150.038.37462652
CTCGTAT228950.038.09356343
CGTATGC243750.036.14859845
ATCTCGT240250.036.1151841
CAATCTC245850.035.15221439
ACCAATC247200.034.9881537
TCGTATG254100.034.77123344
CCAATCT250300.034.6099638
AATCTCG250950.034.54780240
GACCAAT254850.034.23571436
TCTGCTT257850.034.18526557
Produced by FastQC (version 0.11.2)