FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n01_ds774-005-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n01_ds774-005-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6180164
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1607732.6014358195025244RNA PCR Primer, Index 24 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG300250.040.36556269
GACGTGT101250.038.854841
GCTTGAA330050.036.61644760
TGCTTGA337350.035.60933759
CTGCTTG342600.035.03373758
CTTGAAA362550.033.486361
GAAAAAA371050.033.14690464
TGAAAAA383900.031.87563963
TTGAAAA383050.031.73021562
CTTCTGC381950.031.55087555
TCTGCTT382850.031.48571857
CGTCTTC388750.031.00786252
GTCTTCT396650.030.25112253
TTCTGCT398100.030.1322756
TCTTCTG403850.029.8570254
CCGTCTT440300.027.37726451
GTGACTG12050.026.9200651
GCCGTCT448050.026.85751350
ACGTGTG161500.025.3423841
TGACTGG12800.025.3380012
Produced by FastQC (version 0.11.2)