FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n01_ds395-5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n01_ds395-5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13222632
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1215880.9195446110880193TruSeq Adapter, Index 6 (100% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA308700.029.34769660
TGCTTGA327550.027.40599659
CTTGAAA363600.025.15369461
CTGCTTG359400.024.97746858
AAAAAAG368700.024.75897269
GTGACTG33000.023.6337131
TGAAAAA416050.022.1567963
GAAAAAA422350.021.9814964
GACTGGA37050.021.6040613
TTGAAAA432450.021.36439362
TGACTGG38750.020.5675452
GACGTGT78600.019.6255761
TCTGCTT465600.019.5099957
GGAGTTC44100.018.462597
CTTCTGC511050.017.75462555
TTCTGCT519750.017.55036456
CCGTCTT549500.016.34892751
CTGGAGT49900.016.24755
CGTCTTC559300.016.1489452
TCTTCTG571100.015.93609254
Produced by FastQC (version 0.11.2)