FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n01_ds395-023-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n01_ds395-023-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16456272
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG272135216.53686813149418TruSeq Adapter, Index 16 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG374310.2274573487847065RNA PCR Primer, Index 16 (97% over 43bp)
CACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTC249680.1517233064694118TruSeq Adapter, Index 16 (98% over 54bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA248310.15089079713801523TruSeq Adapter, Index 16 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG243990.14826565822441437TruSeq Adapter, Index 16 (98% over 63bp)
CGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTT223530.13583270864749927TruSeq Adapter, Index 16 (98% over 56bp)
AGAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA215260.13080726910687915RNA PCR Primer, Index 16 (100% over 43bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTG212050.12885664505302297TruSeq Adapter, Index 16 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC205080.12462117787066232TruSeq Adapter, Index 16 (98% over 63bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA176950.10752739138001609TruSeq Adapter, Index 16 (98% over 63bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGA176580.10730255309343453TruSeq Adapter, Index 16 (98% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG3331350.063.5832169
GACGTGT497600.054.183761
GAAAAAA4030800.053.76437464
GAATCGG44950.050.8901442
TGCCGTG68500.049.05507769
TGAAAAA4722050.045.69812863
TTGAAAA4705100.045.59952562
CTTGAAA4726750.045.3636661
AGAATCG51400.044.7139241
GCTTGAA4793000.044.6171460
TGCTTGA5004500.042.6005559
CTGCTTG5173800.041.1898458
TCTGCTT5361600.039.756157
AAAAAGG229800.039.5740969
TTCTGCT5380900.039.54426256
CTTCTGC5475400.039.03693455
TCTTCTG5543800.038.48994454
GTCTTCT5592800.038.17801753
AGACGGA35800.038.0755121
CGTCTTC5742950.037.2333152
Produced by FastQC (version 0.11.2)