FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n01_ds131-05-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n01_ds131-05-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9438473
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG862500.9138130712457406TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG183550.035.4490958
TGCTTGA193300.033.75010359
GCCGTCT194350.033.55001450
CCGTCTT200000.032.75747751
TGCCGTC202050.032.3909749
GCTTGAA207000.032.13306460
ATGCCGT205600.031.8988148
CGTCTTC215500.030.3855252
TATGCCG217400.030.21501747
GTATGCC225700.029.19559146
TCTGCTT228650.028.77380257
GACGTGT30650.028.1469171
CTTCTGC234600.028.07344455
GTCTTCT242850.027.20498353
CGTATGC249700.026.3341845
TCTTCTG254400.025.84779754
TTCTGCT255700.025.79733856
CTTGAAA262600.025.64487361
TCGTATG270400.024.27993644
TATCTCG269550.024.2541140
Produced by FastQC (version 0.11.2)