FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n01_ds131-005-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n01_ds131-005-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10346563
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG2188982.115659084084251RNA PCR Primer, Index 23 (100% over 43bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT143700.043.4657671
AAAAAAG415150.041.75874369
GCTTGAA451400.038.35941760
TGCTTGA459100.037.4229959
CTGCTTG461250.037.1662758
GAAAAAA505200.034.89586364
CTTGAAA503750.034.52375461
TCACGGC55350.033.65832557
ACGTGTG188600.033.6245042
CACGGCT55650.033.6008758
TCTGCTT514350.033.42994757
GTCACGG61100.033.4269656
CATCTCG69900.032.42683866
TGAAAAA543850.032.30172363
CGTCTTC538750.032.1720552
CGGCTAC57800.031.99288260
CTTCTGC540500.031.94660855
GTCTTCT545600.031.70489953
TTGAAAA550700.031.66186762
TTCTGCT545900.031.56739656