Basic Statistics
Measure | Value |
---|---|
Filename | HVHNYAFXX_n01_ds131-005-2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 10346563 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG | 218898 | 2.115659084084251 | RNA PCR Primer, Index 23 (100% over 43bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GACGTGT | 14370 | 0.0 | 43.465767 | 1 |
AAAAAAG | 41515 | 0.0 | 41.758743 | 69 |
GCTTGAA | 45140 | 0.0 | 38.359417 | 60 |
TGCTTGA | 45910 | 0.0 | 37.42299 | 59 |
CTGCTTG | 46125 | 0.0 | 37.16627 | 58 |
GAAAAAA | 50520 | 0.0 | 34.895863 | 64 |
CTTGAAA | 50375 | 0.0 | 34.523754 | 61 |
TCACGGC | 5535 | 0.0 | 33.658325 | 57 |
ACGTGTG | 18860 | 0.0 | 33.624504 | 2 |
CACGGCT | 5565 | 0.0 | 33.60087 | 58 |
TCTGCTT | 51435 | 0.0 | 33.429947 | 57 |
GTCACGG | 6110 | 0.0 | 33.42696 | 56 |
CATCTCG | 6990 | 0.0 | 32.426838 | 66 |
TGAAAAA | 54385 | 0.0 | 32.301723 | 63 |
CGTCTTC | 53875 | 0.0 | 32.17205 | 52 |
CGGCTAC | 5780 | 0.0 | 31.992882 | 60 |
CTTCTGC | 54050 | 0.0 | 31.946608 | 55 |
GTCTTCT | 54560 | 0.0 | 31.704899 | 53 |
TTGAAAA | 55070 | 0.0 | 31.661867 | 62 |
TTCTGCT | 54590 | 0.0 | 31.567396 | 56 |