FastQCFastQC Report
Wed 28 Mar 2018
HVHNYAFXX_n01_dna2-frb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNYAFXX_n01_dna2-frb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7584019
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG3474064.5807638403859485TruSeq Adapter, Index 9 (100% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG533900.048.7610869
GAAAAAA620650.042.3791864
GACGTGT171000.039.572141
GCTTGAA768300.033.9744160
CTTGAAA788900.033.14848761
TGCTTGA796550.032.68287359
CTGCTTG798850.032.56760858
TGAAAAA839650.031.27230563
TCTGCTT839000.031.0543357
TTGAAAA850000.030.85904762
CTTCTGC849300.030.72646155
TTCTGCT848550.030.72109656
CGTCTTC871900.029.94584852
GTCTTCT872700.029.8867753
TCTTCTG881850.029.60014254
CCGTCTT919600.028.31338951
GCCGTCT919100.028.2987650
TGCCGTC966850.026.9261449
GAATCGG22750.026.8431872
ATGCCGT976950.026.65483348
Produced by FastQC (version 0.11.2)