FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_reproductive_ifn--_red_needle_f10_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_reproductive_ifn--_red_needle_f10_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887648
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGGATTTG51940.5851418580338152No Hit
GTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGG40620.45761382890515157No Hit
GTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCC35570.40072190778326544No Hit
GTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTCATGAA34060.3837106600814737No Hit
GCTAACAACAGTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTC22330.25156368290133024No Hit
GCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTG20810.23443977792999027No Hit
CAGTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTCATG19350.21799181657594No Hit
GTATTACCTGACCATGAACAATAAGCATTGGTTGGTGCACAAAGAGTGGT18450.20785266231659397No Hit
GTCAATATGCTAAAACGCGGAGTAGCCCGTGTAAACCCCTTGGGAGGTTT18290.20605014600382135No Hit
GGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTCATGAAAAACCCAAA18160.20458560149969354No Hit
GGTAATACAGATCTGAAAAGTCAAGGCCTGTCCTTGGTTCACAGTCAAGT17570.1979388225963445No Hit
GGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAA17560.1978261653267962No Hit
ATCTAAGCTTGTCCATTTTTAGGCGGCATTTCAAGTGGCCAGAGAACAGC17460.1966995926313133No Hit
GGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCT17440.19647427809221674No Hit
CCTTAAACCTGTCTTGTAACCTTGATACTTACTTTCAGCCTCCATGTGTC17280.1946717617794441No Hit
CAACAGTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTC17090.1925312736580266No Hit
ATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCG16910.19050344280615739No Hit
CTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGA16600.1870110674501604No Hit
ATGCTGGATGAGGGAGTGGAACCAGATGATGTCGATTGCTGGTGCAACAC16570.18667309564151557No Hit
GTCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGCATAGGAGTGG16030.18058960308590793No Hit
CAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGC15710.17698457046036267No Hit
ATGCTAAAACGCGGAGTAGCCCGTGTAAACCCCTTGGGAGGTTTGAAGAG15090.16999981974836872No Hit
CTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTA14920.1680846461660478No Hit
TTCATATCCTGTATCATTGACAATCATCCCGCTATGCTGGGAGCCATGCA14440.1626770972277299No Hit
GTATATGACTTTTTGGCTCGTCGAGCTTCCCAAAAGCCAGGCAATGGCAA14200.15997332275857096No Hit
GAGTAGCCCGTGTAAACCCCTTGGGAGGTTTGAAGAGGTTGCCAGCCGGA13670.1540024874725116No Hit
TCTTAGAACACGTAAACTTGGCACATGTCACCAAGCTCCCTTTGCCAAAA13070.14724305129961426No Hit
GTCCATGACCCAGCAGAAGTCCGGCTGGCAACCTCTTCAAACCTCCCAAG13060.14713039403006598No Hit
GTGTAAACCCCTTGGGAGGTTTGAAGAGGTTGCCAGCCGGACTTCTGCTG12880.14510256317819675No Hit
ATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTCATGAAAA12590.14183550236129636No Hit
TAATAAAGAAGTTCAAGAAAGATCTTGCTGCCATGTTGAGAATAATCAAT12400.13969501423987887No Hit
TCCGTACACAACCCAAGTTGATGTCGTGTTGCACCAGCAATCGACATCAT12310.13868109881394428No Hit
GAGTTATGAGTGCCCTATGCTGGATGAGGGAGTGGAACCAGATGATGTCG12250.13800515519665454No Hit
AGTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTCATGA12210.1375545261184614No Hit
TCCTTGTAGAGTGAGAAGGGAGCGTCACGGCTCTTCTAGATCGCCGTGCC12190.1373292115793648No Hit
GTCTTTAGCTGGTCCACTCAGCCACCACAACACCAGAGAGGGTTACAGAA12180.1372165543098165No Hit
GTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACAT12100.1363152961534302No Hit
CCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATT11830.13327354987562637No Hit
GAATATGACACGCCCTTCAATCTAAGCTTGTCCATTTTTAGGCGGCATTT11760.13248494898878835No Hit
CTCAATATGTCTGCAAAAGAACATTAGTGGACAGAGGTTGGGGAAACGGT11750.13237229171924006No Hit
GTAGTATGCACTCCCGCGTCTAGTGATCTCTGCTGCCATGGCTGTAGTCA11700.13180900537149862No Hit
CGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAA11610.13079508994556402No Hit
CCCCTTGGGAGGTTTGAAGAGGTTGCCAGCCGGACTTCTGCTGGGTCATG11520.1297811745196294No Hit
TCCTAGAGGAGAATGGAGTTCAACTGACAGTTGTTGTGGGATCTGTAAAA11300.12730271458956702No Hit
CTCCAAGAGAGAAGTGGTCCATATGATCGGTTGACCCCGCTGTCACCATT11210.12628879916363245No Hit
GGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAG11020.12414831104221494No Hit
GTTAGTAACATGGCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATC11000.12392299650311836No Hit
CTTCTAGATCGCCGTGCCTCACCTTTTTTGTGATGACAGGTTCCGTACAC10990.12381033923357006No Hit
GTGCGTGGCACGGCCATTGCTCGAATTGCCAACCAGGCCAAAGCAAATCC10940.12324705288582861No Hit
CATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCCTCACCTTGTGTTGGG10530.11862810483434875No Hit
GTCATGGACCGTCAATCACGCTTTCCGCCGCCTGACGCGTCTCCGCCAGC10330.11637495944338296No Hit
GAAGAAATCCGGAGGATTCCGGATTGTCAATATGCTAAAACGCGGAGTAG10310.11614964490428638No Hit
GTACAAGTACCATCCTGACTCCCCCCGCAGATTGGCAGCAGCAGTCAAGC10290.1159243303651898No Hit
CTACAAGGAAGTTGCAAACGCGGTCGCAGACCTGGTTAGAATCAAGAGAA10270.11569901582609322No Hit
ATCTAGAAGAGCCGTGACGCTCCCTTCTCACTCTACAAGGAAGTTGCAAA10240.11536104401744837No Hit
TTATAGATGAATACCCCCGTGCCACATCTCGTTTCCTTTTTTGAGAAGTC10220.1151357294783518No Hit
TCCCTTAACAGCTGTTCCTATGACGGCTGGGTCACATTCTAATGAGTAAT10130.11412181405241718No Hit
TCCATGCTCTGTGCTCAAGCGGACATTCCTTCAGTGTGTCACCGTCGACA10100.1137838422437723No Hit
TTAGTGGACAGAGGTTGGGGAAACGGTTGTGGACTTTTTGGCAAAGGGAG10070.11344587043512744No Hit
ACTCTATGTCAACTGTTGGCTTGTCCTGTGCCATCACGGTAACGCAGCCT10030.11299524135693428No Hit
GTACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGAGTTATGA9910.1116433541223548No Hit
GTGCAGGAGGGGTTGAAGAAGAGAATGACCACAAAGATCATCATGAGCAC9870.11119272504416164No Hit
GCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTACTCCATCTGTCCA9810.11051678142687191No Hit
AGCTTAGATTGAAGGGCGTGTCATATTCCTTGTGCACTGCGGCATTCACA9780.11017880961822706No Hit
TCTCTCCACAGAGGGCCCACCTGATTGAGATGAAAACATGTGAATGGCCA9780.11017880961822706No Hit
TTCTTACATTGTCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGC9750.10984083780958218No Hit
GTCATATTCCTTGTGCACTGCGGCATTCACATTCACCAAGGTCCCAGCTG9620.10837629330545441No Hit
ATATCGGACATGGCTTCGGACAGTCGTTGCCCAACACAAGGTGAGGCCTA9590.10803832149680954No Hit
TCTCCATTCCATACCAGCAGCCGTCTTTTGCTCGAAACGATAGTGGGGGC9500.10702440607087493No Hit
GTTCTAAGAAGATGACCGGGAAGAGCATTCAACCGGAAAATCTGGAGTAT9390.10578517610584377No Hit
GTTGAGAATAATCAATGCTAGGAAAGAGAGGAAGAGACGTGGCGCAGACA9360.1054472042971989No Hit
GTCCACAATGTGTGAGACTTTGGCCATTCACATGTTTTCATCTCAATCAG9350.10533454702765059No Hit
ATCATGACCAAGTATATGACTTTTTGGCTCGTCGAGCTTCCCAAAAGCCA9300.10477126067990916No Hit
TCTCACAGTGGCCTCAAATGCCTTTCCGATGGTGCTACCACTCCTATGCC9290.10465860341036086No Hit
CTCTTGGAGTGCTTGTGATTCTACTCATGGTGCAGGAGGGGTTGAAGAAG9240.10409531706261942No Hit
CACTAGCAGCGTGCCTATGAGGATCTGTGAGAACCAGGACATTCCTCCAA9190.10353203071487796No Hit
TTCCTTCAGTGTGTCACCGTCGACAACAAAACTGTTGTTGGTCTTTGCCG9000.10139154259346046No Hit
ATCCAGCATAGGGCACTCATAACTCATGGTGGCGTCACACATGTGCCCGA9000.10139154259346046No Hit
CTCCTGCACCATGAGTAGAATCACAAGCACTCCAAGAGAGAAGTGGTCCA8910.10037762716752588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGTG2600.085.821931
AGTGCGT2600.085.8074344
TTCGAGA6250.085.214352
GTTCGAG13900.079.747281
CAGTGCG2850.078.2804643
GCGACAG3350.075.202651
CGAGTCT7800.074.7355044
TTCGAGT7850.074.2636642
TCGAGTC8000.072.867113
TCGAGAT7400.071.967523
GAGATGC7550.068.631285
GAGTCTG8550.067.3380365
CGACGGA554.244268E-665.421339
AGATGCT8250.062.8080146
GTGCGTG4700.059.727821
GTGGCGA1400.056.545915
GCGACGG651.1427293E-555.3565148
TGCGTGG4850.054.9061472
AGTCTGA13000.054.8152961
CGACAGT4700.053.5958562
Produced by FastQC (version 0.11.2)