FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_pr8_cb10-14_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_pr8_cb10-14_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231734
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG9800.4228986683007241No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA8070.34824410746804524No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT5860.25287614247369833No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG5190.22396368249803655No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT5190.22396368249803655No Hit
GCCACTGCTCCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCAT4140.1786531108943875No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC3310.1428361828648364No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA3240.1398154780912598No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT3160.1363632440643151No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT3160.1363632440643151No Hit
GCATCCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTT3030.13075336377053No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2770.11953360318295977No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT2730.11780748616948744No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA2710.11694442766275125No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2590.11176607662233423No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA2570.11090301811559806No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA2540.1096084303554938No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2530.1091769011021257No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT2390.10313549155497252No Hit
GCATTATATGTCCAAATGTCCAGAAATCCATCATCAACTTTTTTATTTAA2370.10227243304823633No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT2350.10140937454150016No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2340.10097784528813208No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACTC100.0072419574143.957236
AATACTA202.2049098E-6143.957232
GAGCGAA1050.0130.247021
GCAAAAG7150.093.6225363
GCAGGGT3950.091.1121759
AGCAGGG7200.074.977738
AGCAAAA8950.074.793432
AGCGAAA1850.073.923992
AAGCAGG11150.072.301397
TTAGTAG300.001990024571.978621
GAGCAAA9550.070.848061
GCAGGGG2450.070.509669
CAAAAGC10200.065.627564
TAGTAGA350.003666383761.6959572
CTATTCG502.0798904E-457.5828979
ACTATTT651.1386581E-555.3681685
AGCAGGC651.1386581E-555.3681688
TAAACTA553.3304046E-452.3480835
TACTATT553.3304046E-452.3480834
CCAGCTC901.3864028E-647.9857443
Produced by FastQC (version 0.11.2)