FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_ny238_plasmid_mix_cb10-14_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_ny238_plasmid_mix_cb10-14_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613395
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT10420.1698742246024177No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT10270.16742881829816025No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG9970.16253800568964533No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG8930.14558318864679368No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG8400.13694275303841733No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA8220.1340082654733084No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG7670.12504177569103106No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT7630.12438966734322908No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT7390.12047701725641714No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC7140.11640134008265472No Hit
TCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTG6920.11281474416974381No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT6850.11167355456109032No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT6550.10678274195257541No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA6460.10531549817002095No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG6430.10482641690916945No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT6370.10384825438746648No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC6370.10384825438746648No Hit
GGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG6310.1028700918657635No Hit
TTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCC6190.10091376682235755No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAT259.661828E-486.3656544
GTGCTAG300.001991101871.9831161
TGTGTAT350.003674286961.6746679
CCATATT1051.1823431E-954.835344
GTATGAA400.006231484553.9697347
TTCCTAT952.0165971E-645.4630241
CCTATAC952.0188636E-645.455613
GCGGTCT855.5826633E-542.3257529
CATATTA1401.5072146E-841.1265075
ATACATC1252.8772047E-740.303973
CTATACA1105.532109E-639.257124
TATAAGT1001.4506007E-435.991562
TGGGCCC1001.451993E-435.985696
GAGCGTT800.002112992635.976899
TTTAAAC1051.9342922E-434.2720873
CTTGTGC850.00284469533.8606038
TTGGTCC1102.545588E-432.7062649
TATATAT4250.032.1806872
TTAAGGT900.003759472631.987284
GAATATA1153.296116E-431.2970071
Produced by FastQC (version 0.11.2)